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If generating v2 sample sheets for devices other than those listed, affirm the final sample sheet has the i5 index in the correct orientation as described above.
Investigate how AI is reworking genomic investigate, driving scientific insights, and enabling multiomic discovery
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Check out the Illumina sequencing workflow in 3-dimensional depth. Go from sample preparing to cluster generation, sequencing over a movement mobile with the proprietary SBS course of action, and information Investigation.
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For Nearby Operate Manager (LRM), input the sequences from the reverse complement when establishing the operate Therefore the software program mechanically generates the ahead orientation as necessary for analysis. This is often the other of most of our direction and is just for the MiniSeq Speedy kits.
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When assessing index sets for coloration harmony, the sequence noticed via the instrument needs to be assessed, whatever the orientation used for the sample sheet. With the i7 index, generally asses shade stability using the "i7 bases for sample sheet" column orientation.
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